Pex19p-dependent Targeting of Pex17p, a Peripheral Component of the Peroxisomal Protein Import Machinery

Pex19p is required for the topogenesis of peroxisomal membrane proteins (PMPs). Here we have demonstrated that Pex19p is also required for the peroxisomal targeting and stability of Pex17p, a peripheral component of the docking complex of the peroxisomal protein import machinery. We have demonstrate...

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Bibliographic Details
Published in:The Journal of biological chemistry 2006-07, Vol.281 (28), p.19417-19425
Main Authors: Girzalsky, Wolfgang, Hoffmann, Linda S., Schemenewitz, Andreas, Nolte, Andreas, Kunau, Wolf-Hubert, Erdmann, Ralf
Format: Article
Language:English
Subjects:
Base Sequence
Binding Sites
Carrier Proteins - metabolism
Cell Membrane - metabolism
Cytosol - metabolism
Membrane Proteins - metabolism
Membrane Transport Proteins
Molecular Sequence Data
Peroxins
Peroxisomes - chemistry
Protein Transport
Repressor Proteins - metabolism
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Signal Transduction
Two-Hybrid System Techniques
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Summary:Pex19p is required for the topogenesis of peroxisomal membrane proteins (PMPs). Here we have demonstrated that Pex19p is also required for the peroxisomal targeting and stability of Pex17p, a peripheral component of the docking complex of the peroxisomal protein import machinery. We have demonstrated that Pex17p is associated with the peroxisomal Pex13p-Pex14p complex as well as with Pex19p. We have identified the corresponding binding sites for Pex14p and Pex19p and demonstrated that a specific loss of the Pex19p interaction resulted in mistargeting of Pex17p. We have shown that a construct consisting only of the Pex19p- and Pex14p-binding sites of Pex17p is sufficient to direct an otherwise cytosolic reporter protein to the peroxisomal membrane in a Pex19p-dependent manner. Our data show that the function of Pex19p as chaperone or import receptor is not restricted to integral membrane proteins but may also include peripheral PMPs. As a consequence of our data, the previous definition of a targeting signal for PMPs (mPTS) as a Pex19p-binding motif in conjunction with a transmembrane segment should be extended to regions comprising a Pex19p-binding motif and a peroxisomal anchor sequence.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M603344200