Recovery of Stem Cells from Cryopreserved Periodontal Ligament

Human post-natal stem cells possess a great potential to be utilized in stem-cell-mediated clinical therapies and tissue engineering. It is not known whether cryopreserved human tissues contain functional post-natal stem cells. In this study, we utilized human periodontal ligament to test the hypoth...

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Bibliographic Details
Published in:Journal of dental research 2005-10, Vol.84 (10), p.907-912
Main Authors: Seo, B.-M., Miura, M., Sonoyama, W., Coppe, C., Stanyon, R., Shi, S.
Format: Article
Language:English
Subjects:
Adult
Animals
Cell Differentiation - physiology
Cell Survival - physiology
Cells, Cultured
Cryopreservation
Dental Cementum - cytology
Dentistry
Humans
Mice
Mice, Nude
Molar, Third
Multipotent Stem Cells - cytology
Multipotent Stem Cells - physiology
Osteoblasts - cytology
Periodontal Ligament - cytology
Statistics, Nonparametric
Stem Cell Transplantation
Transplantation, Heterologous
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Summary:Human post-natal stem cells possess a great potential to be utilized in stem-cell-mediated clinical therapies and tissue engineering. It is not known whether cryopreserved human tissues contain functional post-natal stem cells. In this study, we utilized human periodontal ligament to test the hypothesis that cryopreserved human periodontal ligament contains retrievable post-natal stem cells. These cryopreserved periodontal ligament stem cells maintained normal periodontal ligament stem cell characteristics, including expression of the mesenchymal stem cell surface molecule STRO-1, single-colony-strain generation, multipotential differentiation, cementum/periodontal-ligament-like tissue regeneration, and a normal diploid karyotype. Collectively, this study provides valuable evidence demonstrating a practical approach to the preservation of solid-frozen human tissues for subsequent post-natal stem cell isolation and tissue regeneration. The present study demonstrates that human post-natal stem cells can be recovered from cryopreserved human periodontal ligament, thereby providing a practical clinical approach for the utilization of frozen tissues for stem cell isolation.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910508401007