Direct and indirect effects of hedgehog pathway activation in the mammalian retina

The morphogen Sonic hedgehog (Shh) is expressed by the projection neurons of the retina, retinal ganglion cells (RGCs) and promotes retinal precursor cell (RPC) proliferation. To distinguish between direct and indirect effects of Hedgehog (Hh) pathway activation in the perinatal mouse retina, we fol...

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Bibliographic Details
Published in:Molecular and cellular neuroscience 2006-07, Vol.32 (3), p.274-282
Main Authors: Yu, Chuan, Mazerolle, Chantal J., Thurig, Sherry, Wang, Yaping, Pacal, Marek, Bremner, Rod, Wallace, Valerie A.
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Hedgehog Proteins
Humans
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
NIH 3T3 Cells
Receptors, G-Protein-Coupled - biosynthesis
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - physiology
Retina - growth & development
Retina - metabolism
Retina - physiology
Signal Transduction - physiology
Smoothened Receptor
Trans-Activators - genetics
Trans-Activators - metabolism
Trans-Activators - physiology
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Summary:The morphogen Sonic hedgehog (Shh) is expressed by the projection neurons of the retina, retinal ganglion cells (RGCs) and promotes retinal precursor cell (RPC) proliferation. To distinguish between direct and indirect effects of Hedgehog (Hh) pathway activation in the perinatal mouse retina, we followed the fate of cells that expressed a constitutively active allele of Smoothened (SMO-M2), the signal transduction component of the Hh pathway. SMO-M2 expression promoted a cell-autonomous increase in CyclinD1 expression and RPC proliferation and promoted the development of cells with an inner nuclear layer identity. SMO-M2 expression also inhibited rhodopsin expression in uninfected cells, thus highlighting an unexpected non-cell autonomous effect of Hh pathway activation on photoreceptor development.
ISSN:1044-7431
1095-9327
DOI:10.1016/j.mcn.2006.05.002