Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform

We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allo...

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Bibliographic Details
Published in:Optics letters 2005-09, Vol.30 (17), p.2266-2268
Main Authors: BURKHARDT, Markus, HEINZE, Katrin G, SCHWILLE, Petra
Format: Article
Language:English
Subjects:
Equipment Design
Equipment Failure Analysis
Exact sciences and technology
Feasibility Studies
Image Enhancement - instrumentation
Image Enhancement - methods
Image Interpretation, Computer-Assisted - instrumentation
Image Interpretation, Computer-Assisted - methods
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Optical instruments, equipment and techniques
Physics
Refractometry - instrumentation
Refractometry - methods
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
Visible and ultraviolet spectrometers
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Description
Summary:We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complexbiomolecular dynamics on a single molecule level.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.30.002266