Rapid simultaneous cloning of drug targets from multiple mammalian species

A method for the routine, rapid and simultaneous cloning of drug targets from multiple mammalian species is described. This expedites the generation of recombinant proteins and cell lines that can provide alternatives to animal experiments. This was achieved by the collection of RNA from a comprehen...

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Bibliographic Details
Published in:Alternatives to laboratory animals 2006-06, Vol.34 (3), p.295-303
Main Authors: Jupp, R, Dusanjh, P.K, Walding, A, McHale, M, Belfield, G.P, Delaney, S.J
Format: Article
Language:English
Subjects:
Animal Testing Alternatives - methods
animal use alternatives
Animals
Biological and medical sciences
Cloning, Molecular - methods
complementary DNA
Drug Evaluation, Preclinical - methods
Humans
in vitro studies
laboratory animals
Medical sciences
nonanimal tests
Receptors, Chemokine - chemistry
Receptors, Chemokine - genetics
Receptors, CXCR3
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA - chemistry
RNA - genetics
screening
species differences
Toxicology
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Summary:A method for the routine, rapid and simultaneous cloning of drug targets from multiple mammalian species is described. This expedites the generation of recombinant proteins and cell lines that can provide alternatives to animal experiments. This was achieved by the collection of RNA from a comprehensive range of tissues from a variety of species, and the optimisation of cDNA synthesis. This "zooplate" has been successfully used for the simultaneous amplification and cloning of drug targets from multiple species. These products have subsequently been used to develop in vitro assays that support efficacy and safety studies in new drug discovery programmes. Within the framework of the Three Rs, these reagents can reduce the number of animals required to provide material for ex vivo assays and can refine the in vivo studies that are still necessary.
ISSN:0261-1929
2632-3559
DOI:10.1177/026119290603400310