Nucleolar localization of potato leafroll virus capsid proteins

Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also loca...

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Bibliographic Details
Published in:Journal of general virology 2005-10, Vol.86 (10), p.2891-2896
Main Authors: Haupt, S, Stroganova, T, Ryabov, E, Kim, S.H, Fraser, G, Duncan, G, Mayo, M.A, Barker, H, Taliansky, M
Format: Article
Language:English
Subjects:
Agrobacterium
Biological and medical sciences
Capsid - metabolism
cell nucleolus
coat proteins
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Viral
green fluorescent protein
Green Fluorescent Proteins
immunocytochemistry
Luteovirus - genetics
Luteovirus - metabolism
Microbiology
Miscellaneous
Nicotiana benthamiana
plant viruses
Potato leafroll virus
recombinant proteins
Rhizobium - virology
Solanum tuberosum - virology
Virology
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Summary:Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting the nucleolar localization signal (NoLS). Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5-GFP lost its nucleolar localization in the presence of replicating PLRV.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.81101-0