Cerebral blood flow and morphological changes after hypoxic-ischaemic injury in preterm lambs

To evaluate the effect of cerebral hypoxia-ischaemia induced by partial occlusion of the umbilical cord on the relationship of the regional cerebral blood flow and the cerebral cell death in near-term fetal lambs. Fifteen near-term lambs were assigned to two hypoxic-ischaemic groups with or without...

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Bibliographic Details
Published in:Acta pædiatrica (Oslo) 2005-07, Vol.94 (7), p.903-911
Main Authors: HILARIO, Enrique, REY-SANTANO, Mari Carmen, GONI-DE-CERIO, Felipe, ALVAREZ, Francisco Jose, GASTIASORO, Elena, MIELGO, Victoria Eugenia, CABALLERO, Amale, VALLS-I-SOLER, Adolfo, GOMEZ-URQUIJO, Sonia, ALVAREZ, Antonia
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Apoptosis
Biological and medical sciences
Cerebrovascular Circulation
DNA Fragmentation
General aspects
Hypoxia-Ischemia, Brain - pathology
Hypoxia-Ischemia, Brain - physiopathology
In Situ Nick-End Labeling
Medical sciences
Pulmonary Gas Exchange
Sheep
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Summary:To evaluate the effect of cerebral hypoxia-ischaemia induced by partial occlusion of the umbilical cord on the relationship of the regional cerebral blood flow and the cerebral cell death in near-term fetal lambs. Fifteen near-term lambs were assigned to two hypoxic-ischaemic groups with or without life support (3 h), and a healthy one. Hypoxia-ischaemia was induced by partial occlusion of the umbilical cord (60 min). Routine light and electron microscopy, and the TUNEL method for apoptosis were performed. Regional cerebral blood flow was measured by coloured microspheres. Cardiovascular, gas exchange and pH parameters were also evaluated. Both hypoxic-ischaemic groups produced a transient acidosis and a decrease of base excess in comparison to the healthy group. Cortical and cerebellar zones, where the regional cerebral blood flow values were similar to baseline, showed an increased number of oligodendrocyte-like apoptotic cells. In contrast, in the inner zones, where regional cerebral blood flow was increased, the number of apoptotic cells did not increase. Necrotic neurons were observed in the basal nuclei, mesencephalon, pons and deep cerebellar nuclei. Our results suggest that regional cerebral blood flow and the presence of apoptotic cells, 3 h after hypoxic-ischemic injury, are correlated.
ISSN:0803-5253
1651-2227
DOI:10.1080/08035250510031151