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Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets
The digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specifi...
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| Published in: | Insect biochemistry and molecular biology 2006-07, Vol.36 (7), p.561-569 |
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| Main Authors: | , , , , , , , |
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| container_end_page | 569 |
| container_issue | 7 |
| container_start_page | 561 |
| container_title | Insect biochemistry and molecular biology |
| container_volume | 36 |
| creator | Silva, Fátima C.B.L. Alcazar, Alonso Macedo, Leonardo L.P. Oliveira, Adeliana S. Macedo, Francisco P. Abreu, Luiz R.D. Santos, Elizeu A. Sales, Mauricio P. |
| description | The digestive system of
Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific
α-galactosidasic and
β-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of
C. capitata was examined by bioassay.
C. capitata was susceptible to SBTI which affected larval mass at ED
50 3.01%. |
| doi_str_mv | 10.1016/j.ibmb.2006.04.004 |
| format | article |
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Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific
α-galactosidasic and
β-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of
C. capitata was examined by bioassay.
C. capitata was susceptible to SBTI which affected larval mass at ED
50 3.01%.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2006.04.004</identifier><identifier>PMID: 16835021</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acid proteinases ; Animals ; Caseins - pharmacology ; Ceratitis capitata ; developmental stages ; digestive enzymes ; Diptera - enzymology ; Diptera - growth & development ; Disaccharidases ; Electrophoresis, Polyacrylamide Gel ; fruit flies ; Glycoside Hydrolases - metabolism ; insect development ; mortality ; SBTI ; Serine Endopeptidases - metabolism ; Serine proteinases ; soybean trypsin inhibitor ; Tephritidae ; Trypsin Inhibitor, Kunitz Soybean - pharmacology ; trypsin inhibitors ; weight loss</subject><ispartof>Insect biochemistry and molecular biology, 2006-07, Vol.36 (7), p.561-569</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-58f6439d0559bf55e1651355cf640ef178e6093226ef52431d462b527d3935e03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16835021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Fátima C.B.L.</creatorcontrib><creatorcontrib>Alcazar, Alonso</creatorcontrib><creatorcontrib>Macedo, Leonardo L.P.</creatorcontrib><creatorcontrib>Oliveira, Adeliana S.</creatorcontrib><creatorcontrib>Macedo, Francisco P.</creatorcontrib><creatorcontrib>Abreu, Luiz R.D.</creatorcontrib><creatorcontrib>Santos, Elizeu A.</creatorcontrib><creatorcontrib>Sales, Mauricio P.</creatorcontrib><title>Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>The digestive system of
Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific
α-galactosidasic and
β-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of
C. capitata was examined by bioassay.
C. capitata was susceptible to SBTI which affected larval mass at ED
50 3.01%.</description><subject>Acid proteinases</subject><subject>Animals</subject><subject>Caseins - pharmacology</subject><subject>Ceratitis capitata</subject><subject>developmental stages</subject><subject>digestive enzymes</subject><subject>Diptera - enzymology</subject><subject>Diptera - growth & development</subject><subject>Disaccharidases</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>fruit flies</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>insect development</subject><subject>mortality</subject><subject>SBTI</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Serine proteinases</subject><subject>soybean trypsin inhibitor</subject><subject>Tephritidae</subject><subject>Trypsin Inhibitor, Kunitz Soybean - pharmacology</subject><subject>trypsin inhibitors</subject><subject>weight loss</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkcuO1DAQRS0EYpqBH2ABXiFYJJSfSRAb6OEx0kgspmdtOXGlcavzwHa3NPwEv4yjbsEOVparzq26qkvIcwYlA6bf7krfDm3JAXQJsgSQD8iK1VVTAJfwkKyg0apglawvyJMYd5AJqarH5ILpWijgbEV-XfktxuSPSHH8eT9gpO4Q_LilDo-4n-YBx0Snnq4x2OSTj7Szs082Wfr6ys8pl99tcP4ecs9ZfEPt6Cj2PXYpLrrbj5trOo3U56_7syti3oF0DlNCP9qINNmwxRSfkke93Ud8dn4vyd3nT5v11-Lm25fr9YebopNKpELVvZaicaBU0_ZKIdOKCaW6XAbsWVWjhkZwrrFXXArmpOat4pUTjVAI4pK8Os3NFn4csisz-Njhfm9HnA7R6FoLKfOZ_geyRrBKyGUiP4FdmGIM2Js5-MGGe8PALHmZnVnyMkteBqTJaWTRi_P0Qzug-ys5B5SBlyegt5Ox2-CjubvlwAQwqBupFuL9icB8rqPHYGLncezQ-ZBDMG7y_3LwG9o1sIM</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Silva, Fátima C.B.L.</creator><creator>Alcazar, Alonso</creator><creator>Macedo, Leonardo L.P.</creator><creator>Oliveira, Adeliana S.</creator><creator>Macedo, Francisco P.</creator><creator>Abreu, Luiz R.D.</creator><creator>Santos, Elizeu A.</creator><creator>Sales, Mauricio P.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets</title><author>Silva, Fátima C.B.L. ; Alcazar, Alonso ; Macedo, Leonardo L.P. ; Oliveira, Adeliana S. ; Macedo, Francisco P. ; Abreu, Luiz R.D. ; Santos, Elizeu A. ; Sales, Mauricio P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-58f6439d0559bf55e1651355cf640ef178e6093226ef52431d462b527d3935e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acid proteinases</topic><topic>Animals</topic><topic>Caseins - pharmacology</topic><topic>Ceratitis capitata</topic><topic>developmental stages</topic><topic>digestive enzymes</topic><topic>Diptera - enzymology</topic><topic>Diptera - growth & development</topic><topic>Disaccharidases</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>fruit flies</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>insect development</topic><topic>mortality</topic><topic>SBTI</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Serine proteinases</topic><topic>soybean trypsin inhibitor</topic><topic>Tephritidae</topic><topic>Trypsin Inhibitor, Kunitz Soybean - pharmacology</topic><topic>trypsin inhibitors</topic><topic>weight loss</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, Fátima C.B.L.</creatorcontrib><creatorcontrib>Alcazar, Alonso</creatorcontrib><creatorcontrib>Macedo, Leonardo L.P.</creatorcontrib><creatorcontrib>Oliveira, Adeliana S.</creatorcontrib><creatorcontrib>Macedo, Francisco P.</creatorcontrib><creatorcontrib>Abreu, Luiz R.D.</creatorcontrib><creatorcontrib>Santos, Elizeu A.</creatorcontrib><creatorcontrib>Sales, Mauricio P.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, Fátima C.B.L.</au><au>Alcazar, Alonso</au><au>Macedo, Leonardo L.P.</au><au>Oliveira, Adeliana S.</au><au>Macedo, Francisco P.</au><au>Abreu, Luiz R.D.</au><au>Santos, Elizeu A.</au><au>Sales, Mauricio P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>36</volume><issue>7</issue><spage>561</spage><epage>569</epage><pages>561-569</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>The digestive system of
Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific
α-galactosidasic and
β-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of
C. capitata was examined by bioassay.
C. capitata was susceptible to SBTI which affected larval mass at ED
50 3.01%.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16835021</pmid><doi>10.1016/j.ibmb.2006.04.004</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Insect biochemistry and molecular biology, 2006-07, Vol.36 (7), p.561-569 |
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| source | Elsevier |
| subjects | Acid proteinases Animals Caseins - pharmacology Ceratitis capitata developmental stages digestive enzymes Diptera - enzymology Diptera - growth & development Disaccharidases Electrophoresis, Polyacrylamide Gel fruit flies Glycoside Hydrolases - metabolism insect development mortality SBTI Serine Endopeptidases - metabolism Serine proteinases soybean trypsin inhibitor Tephritidae Trypsin Inhibitor, Kunitz Soybean - pharmacology trypsin inhibitors weight loss |
| title | Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets |
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