Quantitation of glutathione S transferase-π in the urine of preterm neonates

• Published in: Pediatrics international 2005-10, Vol.47 (5), p.528-531
• Main Authors: Tsukahara, Hirokazu, Toyo-Oka, Michiyo, Kanaya, Yuko, Ogura, Kazumasa, Kawatani, Masao, Hata, Atsuko, Hiraoka, Masahiro, Mayumi, Mitsufumi
• Format: Article
• Language: English
• Subjects: 8-hydroxy-2′-deoxyguanosine; Acetylglucosaminidase - urine; Albuminuria; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - urine; ELISA; glutathione S transferase-π; Glutathione S-Transferase pi - urine; Humans; Infant, Newborn; Infant, Premature - urine; oxidative stress; preterm neonate
• ISSN: 1328-8067; 1442-200X
• DOI: 10.1111/j.1442-200x.2005.02123.x

Background : Glutathione S transferases (GSTs) are widely distributed enzymes found in highly varying amounts in tissues of the human body. The enzyme GST‐π in urine has been used as a marker of renal distal tubular cell damage. The present study was intended to evaluate urinary excretion of GST‐π and its relationship to other renal markers and to the status of oxidative stress in preterm neonates. Methods : Levels of urinary GST‐π, N‐acetyl‐β‐glucosaminidase (a marker of proximal tubular damage), albumin (a marker of glomerular damage) and 8‐hydroxy‐2′‐deoxyguanosine (a marker of oxidative stress) and serum creatinine were measured in preterm neonates at 1 and 4 weeks of age. Results : The results showed that urinary excretion of GST‐π is increased in preterm neonates compared with reported values for healthy adults. No significant relationship was detected between urinary GST‐π and other markers for renal function. Urinary GST‐π showed significantly positive correlation with urinary 8‐hydroxy‐2′‐deoxyguanosine at 1 and 4 weeks. Sick neonates treated with supplemental oxygen and mechanical ventilation showed significantly higher levels of GST‐π as well as 8‐hydroxy‐2′‐deoxyguanosine than clinically stable neonates did at 4 weeks. Conclusions : These results indicate the potential effect of systemic oxidative stress on urinary excretion of GST‐π. Further studies are necessary to explore the effect of oxidative conditions on expression of GST‐π in distal tubules in the human kidney.