Effects of acitretin on spermatogenesis of rats

• Published in: Journal of the European Academy of Dermatology and Venereology 2006-07, Vol.20 (6), p.689-692
• Main Authors: Sengör, B, Bayramgürler, D, Müezzinoglu, B, Altintas, L, Bilen, N, Apaydin, R
• Format: Article
• Language: English
• Subjects: acitretin; Acitretin - pharmacology; Animals; Dose-Response Relationship, Drug; Fibroblasts - cytology; Fibroblasts - drug effects; Keratolytic Agents - pharmacology; Leydig Cells - cytology; Leydig Cells - drug effects; Male; Rats; Rats, Wistar; retinoid; Seminiferous Tubules - cytology; Seminiferous Tubules - drug effects; spermatogenesis; Spermatogenesis - drug effects; Tretinoin - pharmacology; Vitamin A - pharmacology
• ISSN: 0926-9959; 1468-3083
• DOI: 10.1111/j.1468-3083.2006.01573.x

In this experimental study the possible effects of the acitretin on the spermatogenesis of the rats were investigated histopathologically. Thirty‐nine male adult Wistar albino rats were divided into 3 groups as two experimental groups and one control group. The first group consisting 14 rats were applied orally standard dose (0.75 mg/kg/day) acitretin and the second group consisting 16 rats were applied high dose (1.5 mg/kg/day) acitretin. Acitretin was given within dimetil sulphoxide (DMSO), which was diluted with saline solution as a ratio of 1/10, in order to increase its solubility. The control group consisting 9 rats were given only saline solution including DMSO for 8 weeks. After 8 weeks of the administration, half of the rats in the first and second groups and the entire control group were sacrificed under deep ether anaesthesia and bilateral orchiectomy was made. The remaining rats were compared with the control group using a similar method at the end of 8 weeks of wash‐off period. The orchiectomy materials were histopathologically evaluated under the light microscope for spermatogenesis according to parameters including spermatogenetic activity, spermatogenetic organization, seminiferous tubular diameter, interstitial Leydig cells and fibroblasts. The groups, which were evaluated at the end of the 8th and 16th weeks, were compared with the control group regarding the mentioned parameters and no statistical significance was observed among the groups. In our study it was concluded that the standard and high doses of acitretin do not have any effect on the spermatogenesis of the rats.