The budding yeast protein Chl1p has a role in transcriptional silencing, rDNA recombination, and aging

We show that the budding yeast protein Chl1p, required for sister-chromatid cohesion, also modulates transcriptional silencing at HMR and telomeres. The absence of this protein results in increased silencing at HMR and, conversely, in decreased silencing at the telomere. The regulation of silencing...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-11, Vol.337 (1), p.167-172
Main Authors: Das, Shankar Prasad, Sinha, Pratima
Format: Article
Language:English
Subjects:
Aging
Chl1p
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - physiology
DNA, Ribosomal - genetics
Gene Expression Regulation, Fungal
Gene Silencing
Heat shock
Hot Temperature
Life span
Mutation
rDNA recombination
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - physiology
Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism
Sir proteins
Sister Chromatid Exchange
Sister-chromatid cohesion
Telomere - genetics
Transcription, Genetic
Transcriptional silencing
Yeast
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Summary:We show that the budding yeast protein Chl1p, required for sister-chromatid cohesion, also modulates transcriptional silencing at HMR and telomeres. The absence of this protein results in increased silencing at HMR and, conversely, in decreased silencing at the telomere. The regulation of silencing by Chl1p at these two loci is dependent on the presence of Sir proteins. Chl1p also acts synergistically with Sir2p to suppress rDNA recombination. In the absence of this protein, yeast cells exhibit reduced life span and hypersensitivity to heat stress. These observations suggest a role of Chl1p in regulating chromatin structure.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.09.034