The budding yeast protein Chl1p has a role in transcriptional silencing, rDNA recombination, and aging

• Published in: Biochemical and biophysical research communications 2005-11, Vol.337 (1), p.167-172
• Main Authors: Das, Shankar Prasad, Sinha, Pratima
• Format: Article
• Language: English
• Subjects: Aging; Chl1p; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - physiology; DNA, Ribosomal - genetics; Gene Expression Regulation, Fungal; Gene Silencing; Heat shock; Hot Temperature; Life span; Mutation; rDNA recombination; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - physiology; Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism; Sir proteins; Sister Chromatid Exchange; Sister-chromatid cohesion; Telomere - genetics; Transcription, Genetic; Transcriptional silencing; Yeast
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2005.09.034

We show that the budding yeast protein Chl1p, required for sister-chromatid cohesion, also modulates transcriptional silencing at HMR and telomeres. The absence of this protein results in increased silencing at HMR and, conversely, in decreased silencing at the telomere. The regulation of silencing by Chl1p at these two loci is dependent on the presence of Sir proteins. Chl1p also acts synergistically with Sir2p to suppress rDNA recombination. In the absence of this protein, yeast cells exhibit reduced life span and hypersensitivity to heat stress. These observations suggest a role of Chl1p in regulating chromatin structure.