Glucose Oxidase Multilayer Modified Microcantilevers for Glucose Measurement

We report a novel enzyme-based microcantilever sensor by using layer-by-layer nanoassembly modification. A glucose oxidase (GOx) functionalized microcantilever underwent bending when it was exposed to glucose solutions. The magnitudes of bending were proportional to the concentrations of glucose. Th...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-10, Vol.77 (19), p.6197-6204
Main Authors: Yan, Xiaodong, Xu, Xiaohe Karen, Ji, Hai-Feng
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Exact sciences and technology
General, instrumentation
Glucose - analysis
Glucose - metabolism
Glucose Oxidase - chemistry
Glucose Oxidase - metabolism
Hydrogen Peroxide - metabolism
Protein Conformation
Reproducibility of Results
Temperature
Time Factors
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Summary:We report a novel enzyme-based microcantilever sensor by using layer-by-layer nanoassembly modification. A glucose oxidase (GOx) functionalized microcantilever underwent bending when it was exposed to glucose solutions. The magnitudes of bending were proportional to the concentrations of glucose. The cantilever bending was specific toward glucose, but not to other sugars such as mannose, fructose, or galactose. The flow rate effect on the cantilever bending response is also discussed. The bending mechanism was investigated, and the kinetic and thermodynamic analysis and experimental results showed that the conformational change of GOx and gluconic formation were the origin of cantilever deflection.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac050801q