Vascular Sensitivity to Phenylephrine in Rats Submitted to Anaerobic Training and Nandrolone Treatment

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2005-10, Vol.46 (4, Part 2 Suppl), p.1010-1015
• Main Authors: Cunha, Tatiana Sousa, José, Maria, Moura, Costa Sampaio, Bernardes, Celene Fernandes, Tanno, Ana Paula, Marcondes, Fernanda Klein
• Format: Article
• Language: English
• Subjects: Anabolic Agents - pharmacology; Anaerobiosis; Animals; Antihypertensive agents; Aorta, Thoracic - drug effects; Aorta, Thoracic - physiology; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cardiovascular system; Dose-Response Relationship, Drug; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Enzyme Inhibitors - pharmacology; Experimental diseases; In Vitro Techniques; Lipoproteins, HDL - blood; Lipoproteins, LDL - blood; Male; Medical sciences; Nandrolone - pharmacology; NG-Nitroarginine Methyl Ester - pharmacology; Nitric Oxide Synthase - antagonists & inhibitors; Pharmacology. Drug treatments; Phenylephrine - administration & dosage; Phenylephrine - pharmacology; Physical Conditioning, Animal - physiology; Rats; Rats, Wistar; Vasoconstrictor Agents - administration & dosage; Vasoconstrictor Agents - pharmacology
• ISSN: 0194-911X; 1524-4563; 1524-4563
• DOI: 10.1161/01.HYP.0000174600.51515.e7

The effect of anaerobic physical training and nandrolone treatment on the sensitivity to phenylephrine in thoracic aorta and lipoprotein plasma levels of rats was studied. Sedentary and trained male Wistar rats were treated with vehicle or nandrolone (5 mg/kg IM; twice per week) for 6 weeks. Training was performed by jumping into water (4 sets, 10 repetitions, 30-second rest, 50% to 70% body weight load, 5 days/week, 6 weeks). Two days after the last training session, the animals were killed and blood samples for lipoprotein dosage were obtained. Thoracic aorta was isolated and concentration–effect curves of phenylephrine were performed in intact endothelium and endothelium-denuded aortic rings in the absence or presence of N-l-arginine-methyl ester. No changes were observed in endothelium-denuded aortic rings. However, in endothelium-intact thoracic aorta, anaerobic physical training induced subsensitivity to phenylephrine (pD2=7.11±0.07) compared with sedentary group (7.55±1.74), and this effect was canceled by the inhibition of nitric oxide synthesis. No difference was observed between trained (7.22±0.07) and sedentary (7.28±0.09) groups treated with nandrolone. Anaerobic training induced an increase in high-density lipoprotein levels in vehicle-treated rats, but there were no changes in nandrolone-treated groups. Training associated with nandrolone induced an increase in low-density lipoprotein levels but no change in the other groups. If altering endothelium-dependent vasodilatation is considered to be a beneficial adaptation to anaerobic physical training, it is concluded that nandrolone treatment worsens animalsʼ endothelial function, and this effect may be related to lipoprotein blood levels.