Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase gene

Abstract The bile-resistant, strictly anaerobic bacterium Bilophila wadsworthia is found in human faecal flora, in human infections and in environmental samples. A specific PCR primer set for the gene encoding the first metabolic enzyme in the degradative pathway for taurine in B. wadsworthia, tauri...

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Bibliographic Details
Published in:FEMS microbiology letters 2006-08, Vol.261 (1), p.74-79
Main Authors: Laue, Heike, Smits, Theo H. M., Schumacher, Ulrike K., Claros, Marina C., Hartemink, Ralf, Cook, Alasdair M.
Format: Article
Language:English
Subjects:
Animals
Bacteriological methods and techniques used in bacteriology
Bacteriology
Bile
Bilophila - genetics
Bilophila - isolation & purification
Bilophila wadsworthia
Bilophila wadsworthia isolation
Biological and medical sciences
Biological properties
Biological samples
DNA Primers
Enrichment media
Feces - microbiology
Felidae
Fingerprinting
Flora
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Microbiology
Polymerase Chain Reaction - methods
Primates
Sheep
Taurine
taurine dissimilation
taurine:pyruvate aminotransferase
Transaminases - genetics
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Summary:Abstract The bile-resistant, strictly anaerobic bacterium Bilophila wadsworthia is found in human faecal flora, in human infections and in environmental samples. A specific PCR primer set for the gene encoding the first metabolic enzyme in the degradative pathway for taurine in B. wadsworthia, taurine:pyruvate aminotransferase (tpa), was developed and tested. In addition, enrichment cultures were started from faecal samples of primates and felines and shown to contain B. wadsworthia. These were subcultured on agar media and then identified by PCR fingerprinting. PCR for tpa was successful in all positive enrichment cultures and showed no amplification signal in a variety of other bacterial species. Therefore, this PCR method could be a promising tool for rapid detection of B. wadsworthia in biological samples.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2006.00335.x