Induction of Cell Death in Caco-2 Human Colon Carcinoma Cells by Ellagic Acid Rich Fractions from Muscadine Grapes (Vitis rotundifolia)

Possible anticancer mechanisms exerted by polyphenolic compounds contained in fruits and vegetables include antioxidant activity, the inhibition of proliferation, and the induction of apoptosis in cancer cells. This study examined the effects of four isolated polyphenolic extracts from red muscadine...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-07, Vol.54 (15), p.5336-5343
Main Authors: Mertens-Talcott, Susanne U, Lee, Joon-Hee, Percival, Susan S, Talcott, Stephen T
Format: Article
Language:English
Subjects:
anticarcinogenic activity
Antioxidants - analysis
apoptosis
Apoptosis - drug effects
Biological and medical sciences
Caco-2 Cells
Caspase 3
Caspases - metabolism
Cell Count
Cell Cycle
cell lines
colorectal neoplasms
ellagic acid
Ellagic Acid - analysis
Ellagic Acid - pharmacology
Fermented food industries
Flavonoids - analysis
Food industries
Fruit - chemistry
Fundamental and applied biological sciences. Psychology
grapes
Humans
intestinal mucosa
Kinetics
Phenols - analysis
plant extracts
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polyphenols
Reactive Oxygen Species - metabolism
Vitis - chemistry
Vitis rotundifolia
Wines and vinegars
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Summary:Possible anticancer mechanisms exerted by polyphenolic compounds contained in fruits and vegetables include antioxidant activity, the inhibition of proliferation, and the induction of apoptosis in cancer cells. This study examined the effects of four isolated polyphenolic extracts from red muscadine grapes (Vitis rotundifolia) on vital cell parameters and the induction of apoptosis in Caco-2 colon carcinoma cells. The magnitude of effects in cell culture was then correlated to polyphenolic composition and antioxidant capacity. Whereas anticancer effects of individual polyphenolic compounds have been demonstrated multiple times, information relating to anticancer effects of polyphenolic extracts is not available in abundance. All four extracts induced apoptosis, decreased cell number, and caused alterations in cell cycle kinetics in a concentration-dependent manner. The efficacy of the polyphenolics on vital cell parameters correlated well to the presence of ellagic acid glycosides and flavonoids and also to the antioxidant capacity. This study demonstrated the anticancer properties of ellagic acid rich extracts from red muscadine juice. Keywords: Polyphenols; anticancer; red wine; apoptosis
ISSN:0021-8561
1520-5118
DOI:10.1021/jf060563f