Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas

The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th1/Th2/Th3/Tr1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells...

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Published in:Folia histochemica et cytobiologica 2005-01, Vol.43 (3), p.169-171
Main Authors: Luczyński, Włodzimierz, Kovalchuk, Oksana, Krawczuk-Rybak, Maryna, Mitura-Lesiuk, Małgorzata, Malinowska, Iwona, Chyczewski, Lech, Kowalczyk, Jerzy, Matysiak, Michał
Format: Article
Language:English
Subjects:
Case-Control Studies
Child
Child, Preschool
Gene Expression Regulation
Humans
Interferon-gamma - genetics
Lymphoma - diagnosis
Lymphoma - genetics
Lymphoma - immunology
Lymphoma - pathology
RNA, Messenger - genetics
RNA, Messenger - metabolism
T-Lymphocytes, Helper-Inducer - metabolism
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Summary:The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th1/Th2/Th3/Tr1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4+ and CD8+ cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-gamma, IL-10, IL-4, TGF-beta) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4+ cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.
ISSN:0239-8508
1897-5631