Biomolecular interaction analysis in functional proteomics

To understand the function of highly complex eukaryotic tissues like the human brain, in depth knowledge about cellular protein networks is required. Biomolecular interaction analysis (BIA), as a part of functional proteomics, aims to quantify interaction patterns within a protein network in detail....

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Published in:Journal of Neural Transmission 2006-08, Vol.113 (8), p.1015-1032
Main Authors: Moll, D, Prinz, A, Gesellchen, F, Drewianka, S, Zimmermann, B, Herberg, F W
Format: Article
Language:English
Subjects:
Animals
Biological Assay
Brain - metabolism
Brain Chemistry
Calorimetry
Cercopithecus aethiops
COS Cells
Cyclic AMP - analogs & derivatives
Cyclic AMP-Dependent Protein Kinases - analysis
Energy Transfer
Fluorescence Polarization
Humans
Luminescent Measurements
Proteomics - methods
Surface Plasmon Resonance
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cited_by cdi_FETCH-LOGICAL-c357t-ca910ae4251c25e658a5dd322cf1fa96eb5b3ee768f41654a53f514413f17e453
cites cdi_FETCH-LOGICAL-c357t-ca910ae4251c25e658a5dd322cf1fa96eb5b3ee768f41654a53f514413f17e453
container_end_page 1032
container_issue 8
container_start_page 1015
container_title Journal of Neural Transmission
container_volume 113
creator Moll, D
Prinz, A
Gesellchen, F
Drewianka, S
Zimmermann, B
Herberg, F W
description To understand the function of highly complex eukaryotic tissues like the human brain, in depth knowledge about cellular protein networks is required. Biomolecular interaction analysis (BIA), as a part of functional proteomics, aims to quantify interaction patterns within a protein network in detail. We used the cAMP dependent protein kinase (PKA) as a model system for the binding analysis between small natural ligands, cAMP and cAMP analogues, with their physiological interaction partner, the regulatory subunit of PKA. BIA comprises a variety of methods based on physics, biochemistry and molecular biology. Here we compared side by side real time SPR (surface plasmon resonance, Biacore), a bead based assay (AlphaScreen), a fluorescence based method (Fluorescence polarisation) and ITC (isothermal titration calorimetry). These in vitro methods were complemented by an in cell reporter assay, BRET(2) (bioluminescence resonance energy transfer), allowing to test the effects of cAMP analogues in living cells.
doi_str_mv 10.1007/s00702-006-0515-5
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subjects Animals
Biological Assay
Brain - metabolism
Brain Chemistry
Calorimetry
Cercopithecus aethiops
COS Cells
Cyclic AMP - analogs & derivatives
Cyclic AMP-Dependent Protein Kinases - analysis
Energy Transfer
Fluorescence Polarization
Humans
Luminescent Measurements
Proteomics - methods
Surface Plasmon Resonance
title Biomolecular interaction analysis in functional proteomics
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