Stochastic Modeling of S. cerevisiae Inactivation by Supercritical CO2

Pasteurization of S. cerevisiae in a simple substrate with supercritical CO2 was performed at 36 °C on a laboratory multibatch apparatus of a total volume of 150 mL. The pressure values ranged from 100 to 300 bar. The results show a clear dependence between inactivation ratio and increase of pressur...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology progress 2005-09, Vol.21 (5), p.1461-1465
Main Authors: Spilimbergo, S., Mantoan, D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Carbon Dioxide - administration & dosage
Cell Survival - drug effects
Colony Count, Microbial - methods
Computer Simulation
Fundamental and applied biological sciences. Psychology
Microbial Viability - drug effects
Models, Biological
Models, Statistical
Pressure
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - physiology
Sterilization - methods
Stochastic Processes
Survival Analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pasteurization of S. cerevisiae in a simple substrate with supercritical CO2 was performed at 36 °C on a laboratory multibatch apparatus of a total volume of 150 mL. The pressure values ranged from 100 to 300 bar. The results show a clear dependence between inactivation ratio and increase of pressure. A mathematical modeling of the process was exploited to fit the experimental evidences: inactivation curves were analyzed using a stochastic model based on the multihit model (1). The nonlinear survival curve shows a shoulder and a tail which represent the lag and the resistant phase, respectively. The meaning of the nonlinear relationship between inactivation ratio and time is also discussed; the effect of pressure on the values assumed by the parameters of the model proposed was investigated.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp049554m