The measurement of blood and plasma nitrite by chemiluminescence: Pitfalls and solutions

There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence i...

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Bibliographic Details
Published in:Free radical biology & medicine 2006-08, Vol.41 (4), p.541-548
Main Authors: Pelletier, Mildred M., Kleinbongard, Petra, Ringwood, Lorna, Hito, Rania, Hunter, Christian J., Schechter, Alan N., Gladwin, Mark T., Dejam, André
Format: Article
Language:English
Subjects:
Animals
Chemiluminescence
Data Interpretation, Statistical
Erythrocytes
Free radicals
Humans
Luminescence
Nitric oxide
Nitrite
Nitrites - blood
Plasma
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Summary:There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2006.05.001