Rapid diagnosis and follow up of bladder cancer patients using urinary high molecular weight cytokeratins

We have developed an office-based dot-EIA for the detection of a urinary high molecular weight cytokeratin (CK). Immunohistochemical staining and western blot based on CK1K10 monoclonal antibody were used to identify the CK. Urine of 192 patients with different types, grades, and stages of bladder t...

Full description

Saved in:
Bibliographic Details
Published in:World journal of urology 2006-08, Vol.24 (3), p.345-352
Main Authors: ATTALLAH, Abdelfattah M, SAKR, Hanem A, ISMAIL, Hisham, ISMAIL, Mohamed F, IBRAHIM, Ashraf S, EL-SHARABASY, Mohamed M, EL-DOSOKY, Ibrahim
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies, Monoclonal - immunology
Biological and medical sciences
Bladder cancer
Capillary electrophoresis
Cytokeratin
Diagnosis
Epitopes
Female
Gel electrophoresis
Humans
Immunohistochemistry
Keratins - urine
Male
Medical sciences
Middle Aged
Molecular Weight
Monoclonal antibodies
Neoplasm Staging
Nephrology. Urinary tract diseases
Radiation therapy
Sodium lauryl sulfate
Squamous cell carcinoma
Tumors of the urinary system
Urinary Bladder Neoplasms - diagnosis
Urinary Bladder Neoplasms - pathology
Urinary system involvement in other diseases. Miscellaneous
Urinary tract. Prostate gland
Urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed an office-based dot-EIA for the detection of a urinary high molecular weight cytokeratin (CK). Immunohistochemical staining and western blot based on CK1K10 monoclonal antibody were used to identify the CK. Urine of 192 patients with different types, grades, and stages of bladder tumor and 72 controls were evaluated using dot-EIA. An intense and diffuse cytoplasmic reaction was shown in bladder squamous cell carcinoma. The target epitope was identified in urine at 65, 56, and 40-kDa. The CK purified from urine showed single polypeptide at 65-kDa using SDS-PAGE and single peak at 7.4 min using capillary zone electrophoresis. The dot-EIA detected the CK with high sensitivity (97%) and specificity (94%). The CK was not detected in urine of bladder cancer patients showing response to radiotherapy. The sensitive and specific office-based detection of urinary cytokeratin would be helpful in rapid diagnosis and follow up of bladder carcinoma.
ISSN:0724-4983
1433-8726
DOI:10.1007/s00345-006-0100-4