Distinction of the C-glycosylflavone isomer pairs orientin-isoorientin and vitexin/isovitexin using HPLC-MS exact mass measurement and in-source CID

HPLC-MS using collision induced dissociation (CID) has been utilised for the identification of the C-glycosylflavone isomer pairs orientin/isoorientin and vitexin/isovitexin. HPLC-CID/MS analyses produced pseudo-MS/MS spectra that allowed the identification of the flavone C-glycosides. The efficient...

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Bibliographic Details
Published in:Phytochemical analysis 2005-09, Vol.16 (5), p.295-301
Main Authors: Pereira, C.A.M, Yariwake, J.H, McCullagh, M
Format: Article
Language:English
Subjects:
Apigenin - chemistry
C-glycosylflavone isomers
chemical analysis
chemical structure
Chromatography, High Pressure Liquid
collision induced dissociation
exact mass measurement
flavones
Flavonoids - chemistry
Glucosides - chemistry
glycosides
glycosylation
high performance liquid chromatography
HPLC-MS
Isomerism
isomers
Luteolin - chemistry
Mass Spectrometry
Molecular Structure
Passiflora
Passiflora - chemistry
Passiflora alata
Passiflora caerulea
Passiflora edulis
Passiflora incarnata
Passiflora spp
phytochemicals
plant extracts
Plant Extracts - chemistry
Plants, Medicinal - chemistry
spectral analysis
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Summary:HPLC-MS using collision induced dissociation (CID) has been utilised for the identification of the C-glycosylflavone isomer pairs orientin/isoorientin and vitexin/isovitexin. HPLC-CID/MS analyses produced pseudo-MS/MS spectra that allowed the identification of the flavone C-glycosides. The efficient differentiation of isomers was performed by comparing the CID-MS/MS spectra (including exact mass measurements) of particular fragments from the C-glycoside unit. In order to illustrate some possibilities of these MS techniques, they were applied to the comparative analyses of extracts of Passiflora alata, P. edulis, P. incarnata and P. caerulea (Passifloraceae) that are employed as phytomedicines in Brazil and South America.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.820