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Identification and Functional Characterization of Brainstem Cannabinoid CB₂ Receptors
The presence and function of CB₂ receptors in central nervous system (CNS) neurons are controversial. We report the expression of CB₂ receptor messenger RNA and protein localization on brainstem neurons. These functional CB₂ receptors in the brainstem were activated by a CB₂ receptor agonist, 2-arac...
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| Published in: | Science (American Association for the Advancement of Science) 2005-10, Vol.310 (5746), p.329-332 |
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| Main Authors: | , , , , , , , , , , , , , , |
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| container_end_page | 332 |
| container_issue | 5746 |
| container_start_page | 329 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 310 |
| creator | Van Sickle, Marja D Duncan, Marnie Kingsley, Philip J Mouihate, Abdeslam Urbani, Paolo Mackie, Ken Stella, Nephi Makriyannis, Alexandros Piomelli, Daniele Davison, Joseph S Marnett, Lawrence J Di Marzo, Vincenzo Pittman, Quentin J Patel, Kamala D Sharkey, Keith A |
| description | The presence and function of CB₂ receptors in central nervous system (CNS) neurons are controversial. We report the expression of CB₂ receptor messenger RNA and protein localization on brainstem neurons. These functional CB₂ receptors in the brainstem were activated by a CB₂ receptor agonist, 2-arachidonoylglycerol, and by elevated endogenous levels of endocannabinoids, which also act at CB₁ receptors. CB₂ receptors represent an alternative site of action of endocannabinoids that opens the possibility of nonpsychotropic therapeutic interventions using enhanced endocannabinoid levels in localized brain areas. |
| doi_str_mv | 10.1126/science.1115740 |
| format | article |
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We report the expression of CB₂ receptor messenger RNA and protein localization on brainstem neurons. These functional CB₂ receptors in the brainstem were activated by a CB₂ receptor agonist, 2-arachidonoylglycerol, and by elevated endogenous levels of endocannabinoids, which also act at CB₁ receptors. CB₂ receptors represent an alternative site of action of endocannabinoids that opens the possibility of nonpsychotropic therapeutic interventions using enhanced endocannabinoid levels in localized brain areas.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1115740</identifier><identifier>PMID: 16224028</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Agonists ; Animals ; Arachidonic Acids - pharmacology ; Biological and medical sciences ; Blotting, Western ; Brain stem ; Brain Stem - metabolism ; Cannabinoid Receptor Modulators - metabolism ; Cannabinoids - pharmacology ; Central nervous system ; Cerebellum ; Cerebellum - metabolism ; Cerebral Cortex - metabolism ; Emetics ; Endocannabinoids ; Ferrets ; Immunohistochemistry ; Medical sciences ; Mice ; Neurons ; Neuropharmacology ; Pharmacology. Drug treatments ; Polyunsaturated Alkamides ; Psychodysleptics: hallucinogen ; Psychology. Psychoanalysis. Psychiatry ; Psychopharmacology ; Rats ; Receptor, Cannabinoid, CB2 - agonists ; Receptor, Cannabinoid, CB2 - antagonists & inhibitors ; Receptor, Cannabinoid, CB2 - metabolism ; Receptors ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Spleen ; Vomiting ; Vomiting - prevention & control</subject><ispartof>Science (American Association for the Advancement of Science), 2005-10, Vol.310 (5746), p.329-332</ispartof><rights>Copyright 2005 American Association for the Advancement of Science</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925,33612,33878</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17214502$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16224028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Sickle, Marja D</creatorcontrib><creatorcontrib>Duncan, Marnie</creatorcontrib><creatorcontrib>Kingsley, Philip J</creatorcontrib><creatorcontrib>Mouihate, Abdeslam</creatorcontrib><creatorcontrib>Urbani, Paolo</creatorcontrib><creatorcontrib>Mackie, Ken</creatorcontrib><creatorcontrib>Stella, Nephi</creatorcontrib><creatorcontrib>Makriyannis, Alexandros</creatorcontrib><creatorcontrib>Piomelli, Daniele</creatorcontrib><creatorcontrib>Davison, Joseph S</creatorcontrib><creatorcontrib>Marnett, Lawrence J</creatorcontrib><creatorcontrib>Di Marzo, Vincenzo</creatorcontrib><creatorcontrib>Pittman, Quentin J</creatorcontrib><creatorcontrib>Patel, Kamala D</creatorcontrib><creatorcontrib>Sharkey, Keith A</creatorcontrib><title>Identification and Functional Characterization of Brainstem Cannabinoid CB₂ Receptors</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The presence and function of CB₂ receptors in central nervous system (CNS) neurons are controversial. 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CB₂ receptors represent an alternative site of action of endocannabinoids that opens the possibility of nonpsychotropic therapeutic interventions using enhanced endocannabinoid levels in localized brain areas.</description><subject>Agonists</subject><subject>Animals</subject><subject>Arachidonic Acids - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain stem</subject><subject>Brain Stem - metabolism</subject><subject>Cannabinoid Receptor Modulators - metabolism</subject><subject>Cannabinoids - pharmacology</subject><subject>Central nervous system</subject><subject>Cerebellum</subject><subject>Cerebellum - metabolism</subject><subject>Cerebral Cortex - metabolism</subject><subject>Emetics</subject><subject>Endocannabinoids</subject><subject>Ferrets</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Neurons</subject><subject>Neuropharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyunsaturated Alkamides</subject><subject>Psychodysleptics: hallucinogen</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopharmacology</subject><subject>Rats</subject><subject>Receptor, Cannabinoid, CB2 - agonists</subject><subject>Receptor, Cannabinoid, CB2 - antagonists & inhibitors</subject><subject>Receptor, Cannabinoid, CB2 - metabolism</subject><subject>Receptors</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Spleen</subject><subject>Vomiting</subject><subject>Vomiting - prevention & control</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpFkDlPJDEQRi3ECoYjJkHQCWS969vuEFpcEtJKHCJsVfsAox73YPcES8hP3V-C0Qy7UenTe_pKVQgdEPyTECp_ZRNcNK4EIhTHG2hGcCPqhmK2iWYYM1lrrMQ22sn5FePCGraFtomklGOqZ-jpxro4BR8MTGGMFURbXS6j-QowVO0LJDCTS-F9xUdfnScIMU9uXrUQI_QhjsFW7fnfj4_qzhm3mMaU99APD0N2--u5ix4vLx7a6_r299VNe3Zbe8r5VAuvm4YZDVT3glvChMRSEKWw4qSntqeOCNFbygTRQhMP0oMCIa0GT71lu-h01btI49vS5ambh2zcMEB04zJ3UkutpOJFPFqLy37ubLdIYQ7pT_f9iiKcrAXIBgafIJqQ_3uKEi4wLd7hynvN5dB_nGlOpZQFH6-wh7GD51QqHu8pJgwTzETZxD4BBXyBTQ</recordid><startdate>20051014</startdate><enddate>20051014</enddate><creator>Van Sickle, Marja D</creator><creator>Duncan, Marnie</creator><creator>Kingsley, Philip J</creator><creator>Mouihate, Abdeslam</creator><creator>Urbani, Paolo</creator><creator>Mackie, Ken</creator><creator>Stella, Nephi</creator><creator>Makriyannis, Alexandros</creator><creator>Piomelli, Daniele</creator><creator>Davison, Joseph S</creator><creator>Marnett, Lawrence J</creator><creator>Di Marzo, Vincenzo</creator><creator>Pittman, Quentin J</creator><creator>Patel, Kamala D</creator><creator>Sharkey, Keith A</creator><general>American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20051014</creationdate><title>Identification and Functional Characterization of Brainstem Cannabinoid CB₂ Receptors</title><author>Van Sickle, Marja D ; Duncan, Marnie ; Kingsley, Philip J ; Mouihate, Abdeslam ; Urbani, Paolo ; Mackie, Ken ; Stella, Nephi ; Makriyannis, Alexandros ; Piomelli, Daniele ; Davison, Joseph S ; Marnett, Lawrence J ; Di Marzo, Vincenzo ; Pittman, Quentin J ; Patel, Kamala D ; Sharkey, Keith A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f244t-5f8993c8a28b54d13560651770741b2db2e155bd23518581fa6fa7a56d8af2fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agonists</topic><topic>Animals</topic><topic>Arachidonic Acids - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain stem</topic><topic>Brain Stem - metabolism</topic><topic>Cannabinoid Receptor Modulators - metabolism</topic><topic>Cannabinoids - pharmacology</topic><topic>Central nervous system</topic><topic>Cerebellum</topic><topic>Cerebellum - metabolism</topic><topic>Cerebral Cortex - metabolism</topic><topic>Emetics</topic><topic>Endocannabinoids</topic><topic>Ferrets</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Neurons</topic><topic>Neuropharmacology</topic><topic>Pharmacology. 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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2005-10, Vol.310 (5746), p.329-332 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | American Association for the Advancement of Science; Social Science Premium Collection; Alma/SFX Local Collection; Education Collection |
| subjects | Agonists Animals Arachidonic Acids - pharmacology Biological and medical sciences Blotting, Western Brain stem Brain Stem - metabolism Cannabinoid Receptor Modulators - metabolism Cannabinoids - pharmacology Central nervous system Cerebellum Cerebellum - metabolism Cerebral Cortex - metabolism Emetics Endocannabinoids Ferrets Immunohistochemistry Medical sciences Mice Neurons Neuropharmacology Pharmacology. Drug treatments Polyunsaturated Alkamides Psychodysleptics: hallucinogen Psychology. Psychoanalysis. Psychiatry Psychopharmacology Rats Receptor, Cannabinoid, CB2 - agonists Receptor, Cannabinoid, CB2 - antagonists & inhibitors Receptor, Cannabinoid, CB2 - metabolism Receptors Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Spleen Vomiting Vomiting - prevention & control |
| title | Identification and Functional Characterization of Brainstem Cannabinoid CB₂ Receptors |
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