RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion

Previous work indicates that matrix metalloproteinase-1 (MMP-1) gene expression is a prognostic factor for local recurrence and metastasis in human chondrosarcoma. The current study evaluates the effects of MMP-1 knock-down via RNA interference on chondrosarcoma metastasis in vitro. Of nine siRNA ol...

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Bibliographic Details
Published in:Journal of orthopaedic research 2005-11, Vol.23 (6), p.1467-1474
Main Authors: Yuan, Jun, Dutton, Charyl M., Scully, Sean P.
Format: Article
Language:English
Subjects:
Bone Neoplasms - pathology
Bone Neoplasms - therapy
Cell Line
Chondrosarcoma
Chondrosarcoma - pathology
Chondrosarcoma - therapy
Gene Silencing
Humans
Matrix Metalloproteinase 1 - genetics
Matrix Metalloproteinase Inhibitors
Metastasis
MMP-1
Neoplasm Invasiveness
Plasmids
RNA Interference
RNA, Messenger - analysis
RNA, Small Interfering - therapeutic use
siRNA
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Summary:Previous work indicates that matrix metalloproteinase-1 (MMP-1) gene expression is a prognostic factor for local recurrence and metastasis in human chondrosarcoma. The current study evaluates the effects of MMP-1 knock-down via RNA interference on chondrosarcoma metastasis in vitro. Of nine siRNA oligos tested, one showed a 90% inhibitory effect on MMP-1 gene expression. Stable attenuation of MMP-1 in chondrosarcoma cells was achieved by using a plasmid vector-based siRNA system. Pronounced MMP-1 activity suppression was accompanied by marked inhibition of tumor cell invasion. Cells in which MMP-1 has been stably silenced are useful tools to elucidate the mechanism of MMP-1 mediated tumor metastasis.
ISSN:0736-0266
1554-527X
DOI:10.1016/j.orthres.2005.04.004