Ionic liquid-based aqueous two-phase system, a sample pretreatment procedure prior to high-performance liquid chromatography of opium alkaloids

An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C 4mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium pa...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-11, Vol.826 (1), p.58-62
Main Authors: Li, Shehong, He, Chiyang, Liu, Huwei, Li, Kean, Liu, Feng
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Aqueous two-phase systems
Biological and medical sciences
Chemical Fractionation - methods
Chromatography, High Pressure Liquid - methods
Codeine - isolation & purification
Extraction
Fundamental and applied biological sciences. Psychology
General pharmacology
Imidazoles
Ionic liquid
Medical sciences
Opium alkaloids
Papaver - chemistry
Papaverine - isolation & purification
Pharmacology. Drug treatments
Phosphates
Potassium Compounds
Temperature
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Summary:An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C 4mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium papaveris. To find optimal conditions, the partition behaviors of codeine and papaverine in ionic liquid/salt aqueous two-phase systems were investigated. Various factors were considered systematically, and the results indicated that both the pH value and the salting-out ability of salt had great influence on phase separation. The recoveries of codeine and papaverine were 90.0–100.2% and 99.3–102.0%, respectively, from aqueous samples of P. papaveris by the proposed method.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.08.005