Regulatory role of the 3' untranslated region (3'UTR) of rat 5' deiodinase (D1). effects on messenger RNA translation and stability

The previous findings that both a long and a short type 1 deiodinase (D1) mRNA are present in different tissues and that the D1 gene contains two potential polyA signals suggest that the two mRNAs result from differential polyA signal usage. In this study, we examined the properties of the two D1 mR...

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Bibliographic Details
Published in:Endocrine 2005-08, Vol.27 (3), p.219-225
Main Authors: Arroyo-Helguera, Omar, Mejía-Viggiano, Carmen, Varela-Echavarría, Alfredo, Cajero-Juárez, Marcos, Aceves, Carmen
Format: Article
Language:English
Subjects:
3' Untranslated regions
3' Untranslated Regions - genetics
Animals
Cells, Cultured
Endocrinology
Gene Expression Regulation - physiology
Humans
Iodide peroxidase
Iodide Peroxidase - genetics
Iodide Peroxidase - metabolism
Kidney - cytology
mRNA stability
Poly A - genetics
Protein Biosynthesis - physiology
Rats
RNA Stability - physiology
RNA, Messenger - genetics
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Summary:The previous findings that both a long and a short type 1 deiodinase (D1) mRNA are present in different tissues and that the D1 gene contains two potential polyA signals suggest that the two mRNAs result from differential polyA signal usage. In this study, we examined the properties of the two D1 mRNAs generated in HEK 293 cells by the alternative use of each of the poly A signals in order to ascertain the potential regulatory role of the 3'UTR of this gene. Our results showed that the long mRNA is less stable, but that it is translated more efficiently than the short mRNA. The net result of these differences is a higher D1 activity with the long message. These data suggest that the D1 3'UTR may play an important role in regulating the stability and translational efficiency of the D1 mRNA, both of which could be physiologically relevant when the demand for D1 activity is high.
ISSN:1355-008X
1559-0100
DOI:10.1385/ENDO:27:3:219