Characterization of a cDNA coding for an extracellular calmodulin-binding protein from suspension-cultured cells of Angelica dahurica

• Published in: Planta 2005-10, Vol.222 (3), p.428-437
• Main Authors: Mao, G.H, Hou, L.X, Ding, C.B, Cui, S.J, Sun, D.Y
• Format: Article
• Language: English
• Subjects: abscisic acid; Abscisic Acid - pharmacology; Amino Acid Sequence; amino acid sequences; Amino acids; Angelica; Angelica - drug effects; Angelica - genetics; Angelica - metabolism; Angelica dahurica; Bacteria; Biological and medical sciences; calcium; Calmodulin - metabolism; Calmodulin binding proteins; calmodulin-binding protein; Calmodulin-Binding Proteins - chemistry; Calmodulin-Binding Proteins - genetics; Calmodulin-Binding Proteins - metabolism; Cell growth; cell suspension culture; Cell walls; Cells, Cultured; Complementary DNA; Cultured cells; Cyclopentanes - pharmacology; DNA; DNA, Complementary - genetics; DNA, Plant; Enzymes; Epidermal cells; Fundamental and applied biological sciences. Psychology; gene expression regulation; Gene Expression Regulation, Plant - drug effects; gibberellic acid; Gibberellins - pharmacology; Growth regulators; jasmonic acid; messenger RNA; Metabolism; Molecular Sequence Data; nucleotide sequences; Oxylipins; plant biochemistry; plant genetics; Plant physiology and development; plant proteins; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Protein Binding; Proteins; salicylic acid; Salicylic Acid - pharmacology; Sequence Alignment; Sequence Homology, Amino Acid; signal transduction; transport proteins
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-005-1558-9

In order to characterize a specific extracellular 21-kDa calmodulin-binding protein (named: ECBP21) from Angelica dahurica L. suspension-cultured cells, the cDNA coding for the protein has been cloned. Here, Southern blot analysis shows that there are at least two copies of ECBP21 gene in Angelica genome. Using truncated versions of ECBP21 and synthetic peptide in CaM binding assays, we mapped the calmodulin-binding domain to a 16-amino acid stretch (residues 200-215) at the C-terminal region. The ECBP21 was localized in the cell wall area by the immunogold electron microscopy and by GFP labeling method. These results define ECBP21 as a kind of an extracellular calmodulin-binding protein (CaMBP). Furthermore, using Northern blot analysis, we examined the expression dynamics of ecbp21 during the incubation of Angelica suspension-cultured cells and the treatments with some growth regulators. The above studies further provide the molecular evidence for the existence of the gene coding for extracellular CaMBPs and imply a possible role for ECBP21.