Reduction of polyspermic penetration using biomimetic microfluidic technology during in vitro fertilization

Efforts to improve the in vitro embryo production process in pigs have included modifying culture medium and number of spermatozoa inseminated in order to reduce the incidence of polyspermy. Polyspermy is a pathological condition which results in aberrant embryonic development. The microchannels are...

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Bibliographic Details
Published in:Lab on a chip 2005-01, Vol.5 (11), p.1229-1232
Main Authors: Clark, Sherrie G, Haubert, Kathyrn, Beebe, David J, Ferguson, C Edward, Wheeler, Matthew B
Format: Article
Language:English
Subjects:
Animals
Biomimetics - methods
Female
Fertilization - physiology
Fertilization in Vitro - methods
Male
Microfluidic Analytical Techniques - instrumentation
Microfluidics - instrumentation
Microfluidics - methods
Oocytes - physiology
Spermatozoa - physiology
Swine - embryology
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Summary:Efforts to improve the in vitro embryo production process in pigs have included modifying culture medium and number of spermatozoa inseminated in order to reduce the incidence of polyspermy. Polyspermy is a pathological condition which results in aberrant embryonic development. The microchannels are designed to more closely mimic the function of the oviduct and create a flow pattern of spermatozoa past the oocytes similar to the pattern in the oviduct. In vitro fertilization of porcine oocytes in the microchannels has produced a higher incidence of monospermic penetration (p
ISSN:1473-0197
1473-0189
DOI:10.1039/b504397m