Tissue characterization using high wave number Raman spectroscopy

Raman spectroscopy is a powerful diagnostic tool, enabling tissue identification and classification. Mostly, the so-called fingerprint (∼400-1800 cm ) spectral region is used. application often requires small flexible fiber-optic probes, and is hindered by the intense Raman signal that is generated...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2005-05, Vol.10 (3), p.031116-0311111
Main Authors: Koljenovic´, S, Bakker Schut, T. C, Wolthuis, R, de Jong, B, Santos, L, Caspers, P. J, Kros, J. M, Puppels, G. J
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor - metabolism
bladder
Brain - metabolism
Brain - pathology
Brain Neoplasms - metabolism
brain tumor
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology - instrumentation
fiber-optic probe
high wave number Raman spectroscopy
human tissues
Humans
necrosis
Nerve Tissue Proteins - metabolism
Optical Fibers
Raman mapping
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
Swine
Urinary Bladder - metabolism
Urinary Bladder - pathology
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Summary:Raman spectroscopy is a powerful diagnostic tool, enabling tissue identification and classification. Mostly, the so-called fingerprint (∼400-1800 cm ) spectral region is used. application often requires small flexible fiber-optic probes, and is hindered by the intense Raman signal that is generated in the fused silica core of the fiber. This necessitates filtering of laser light, which is guided to the tissue, and of the scattered light collected from the tissue, leading to complex and expensive designs. Fused silica has no Raman signal in the high wave number region (2400-3800 cm ). This enables the use of a single unfiltered fiber to guide laser light to the tissue and to collect scattered light in this spectral region. We show, by means of a comparison of Raman microspectroscopic maps of thin tissue sections (brain tumors, bladder), measured both in the high wave number region and in the fingerprint region, that essentially the same diagnostic information is obtained in the two wave number regions. This suggests that for many clinical applications the technological hurdle of designing and constructing suitable fiber-optic probes may be eliminated by using the high wave number region and a simple piece of standard optical fiber. ©
ISSN:1083-3668
1560-2281
DOI:10.1117/1.1922307