Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K2...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-11, Vol.337 (3), p.901-907
Main Authors: Kourmouli, Niki, Sun, Yuh-man, van der Sar, Sjaak, Singh, Prim B., Brown, Jeremy P.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Centromere - genetics
Centromere - metabolism
Chromosomal Proteins, Non-Histone - genetics
Epigenesis, Genetic - physiology
Epigenetics
Gene Expression Regulation, Developmental - genetics
Heterochromatin
Heterochromatin - genetics
Heterochromatin - metabolism
Histone code
Histone Code - genetics
Histones - genetics
HP1
Mice
Stem Cells - metabolism
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Summary:We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only “deciphers” the histone code but can also “encode it.”
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.09.132