Reciprocal Interference Between Specific CJD and Scrapie Agents in Neural Cell Cultures

Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune s...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2005-10, Vol.310 (5747), p.493-496
Main Authors: Nishida, Noriuki, Katamine, Shigeru, Manuelidis, Laura
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Brain
Cell culture
Cell Line
Cell lines
Coculture Techniques
Control loops
Creutzfeldt-Jakob disease
Creutzfeldt-Jakob Syndrome
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Humans
Infections
Lesions
Medical sciences
Mice
Neurology
Neurons - metabolism
Neurons - physiology
Pathology
Positive feedback
Prion diseases
Prions - metabolism
Prions - pathogenicity
Prions - physiology
PrPSc Proteins - metabolism
PrPSc Proteins - pathogenicity
Rodents
Scrapie
Sheep
Species Specificity
Superinfection
Virulence
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Summary:Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1118155