Cold-plasma modification of oxide surfaces for covalent biomolecule attachment

While many processes have been developed to modify the surface of glass and other oxides for biomolecule attachment, they rely primarily upon wet chemistry and are costly and time-consuming. We describe a process that uses a cold plasma and a subsequent in vacuo vapor-phase reaction to terminate a v...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2005-11, Vol.21 (5), p.796-801
Main Authors: Larson, B.J., Helgren, J.M., Manolache, S.O., Lau, A.Y., Lagally, M.G., Denes, F.S.
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Biopolymers - analysis
Biopolymers - chemistry
Biosensors
Biotechnology
Coated Materials, Biocompatible - chemistry
Cold plasmas
Cold Temperature
Crystallization - methods
DNA - analysis
DNA - chemistry
Fundamental and applied biological sciences. Psychology
Gases - chemistry
Methods. Procedures. Technologies
Microarray Analysis - instrumentation
Microarray Analysis - methods
Microarrays
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Oxides - chemistry
Surface chemistry
Surface Properties
Various methods and equipments
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Summary:While many processes have been developed to modify the surface of glass and other oxides for biomolecule attachment, they rely primarily upon wet chemistry and are costly and time-consuming. We describe a process that uses a cold plasma and a subsequent in vacuo vapor-phase reaction to terminate a variety of oxide surfaces with epoxide chemical groups. These epoxide groups can react with amine-containing biomolecules, such as proteins and modified oligonucleotides, to form strong covalent linkages between the biomolecules and the treated surface. The use of a plasma activation step followed by an in vacuo vapor-phase reaction allows for the precise control of surface functional groups, rather than the mixture of functionalities normally produced. By maintaining the samples under vacuum throughout the process, adsorption of contaminants is effectively eliminated. This process modifies a range of different oxide surfaces, is fast, consumes a minimal amount of reagents, and produces attachment densities for bound biomolecules that are comparable to or better than commercially available substrates.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2005.02.005