Comparative analysis of the plasmids from two isolates of “ Candidatus Phytoplasma australiense”

Two plasmids from the plant-pathogenic mollicute “ Candidatus Phytoplasma australiense” were completely sequenced from two isolates derived from different plant hosts. Plasmid pPAPh2 (3607 bp) was obtained from Phormium showing Phormium yellow leaf symptoms and pPASb11 (3635 bp) from strawberry show...

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Bibliographic Details
Published in:Plasmid 2006-09, Vol.56 (2), p.138-144
Main Authors: Liefting, Lia W., Andersen, Mark T., Lough, Tony J., Beever, Ross E.
Format: Article
Language:English
Subjects:
Allium cepa
Amino Acid Sequence
Asparagaceae - microbiology
Base Sequence
Candidatus Phytoplasma australiense
Chromosome Mapping
DNA Primers
Fragaria
Fragaria - microbiology
Molecular Sequence Data
Mollicute
Mollicutes
Phytoplasma - genetics
Plant Diseases - genetics
Plasmid
Plasmids - genetics
Rolling-circle replication
Sequence Analysis, DNA
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Summary:Two plasmids from the plant-pathogenic mollicute “ Candidatus Phytoplasma australiense” were completely sequenced from two isolates derived from different plant hosts. Plasmid pPAPh2 (3607 bp) was obtained from Phormium showing Phormium yellow leaf symptoms and pPASb11 (3635 bp) from strawberry showing strawberry lethal yellows symptoms. The plasmids varied in their copy number and nucleotide sequence yet contained the same four open reading frames (ORFs). The deduced amino acid sequence derived from ORF1 shares similarity with hypothetical proteins encoded on the plasmids from onion yellows and beet leafhopper-transmitted virescence agent phytoplasmas. The deduced amino acid sequences of both ORF2 and ORF3 share similarity with functionally unknown proteins on the chromosome of onion yellows phytoplasma. An ORF with a similar sequence to ORF2 is also present on the chromosome of “ Ca. P. australiense.” The deduced amino acid sequence derived from ORF4 is most similar to replication proteins encoded by other phytoplasma plasmids and by geminiviruses, the only protein on the plasmids for which a putative function can be assigned. The identities of the deduced amino acid sequences of ORF1, ORF2, ORF3, and ORF4 between pPAPh2 and pPASb11 were 89, 68, 91, and 68%, respectively; the differences being consistent with the subgroup status of the parental phytoplasmas.
ISSN:0147-619X
1095-9890
DOI:10.1016/j.plasmid.2006.02.001