Incorporation of spin-labelled amino acids into proteins

The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased...

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Bibliographic Details
Published in:Magnetic resonance in chemistry 2005-12, Vol.43 (S1), p.S34-S39
Main Authors: Becker, Christian F. W., Lausecker, Kester, Balog, Mária, Kálai, Tamás, Hideg, Kálmán, Steinhoff, Heinz-Jürgen, Engelhard, Martin
Format: Article
Language:English
Subjects:
Amino Acids - chemical synthesis
Amino Acids - chemistry
chemical synthesis of proteins
Electron Spin Resonance Spectroscopy
EPR spectroscopy
Molecular Structure
protein ligation
Proteins - chemical synthesis
Proteins - chemistry
site-directed spin labelling
Spin Labels
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Summary:The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor‐made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site‐specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site‐specifically spin‐labelled proteins. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0749-1581
1097-458X
DOI:10.1002/mrc.1688