Fed-batch culture under illumination with blue light emitting diodes (LEDs) for astaxanthin production by Haematococcus pluvialis

To increase the cell concentration and the accumulation of astaxanthin, the effects of the fed-batch addition of 10-fold-concentrated medium to supply nutrients, as well as illumination with blue light emitting diodes (LEDs), on cell growth and accumulation of astaxanthin were studied for the cultiv...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2005-09, Vol.100 (3), p.339-342
Main Authors: Lababpour, Abdolmajid, Shimahara, Kazumichi, Hada, Keishi, Kyoui, Yoshiaki, Katsuda, Tomohisa, Katoh, Shigeo
Format: Article
Language:English
Subjects:
ADDITIF AUX ALIMENTS DES ANIMAUX
ADITIVOS DE PIENSOS
astaxanthin
beta Carotene - analogs & derivatives
beta Carotene - biosynthesis
Biological and medical sciences
Biotechnology
Biotechnology - instrumentation
Biotechnology - methods
blue light emitting diode (LED)
CELL CULTURE
Cell Culture Techniques
CHLOROPHYCEAE
Chlorophyta - metabolism
Chlorophyta - radiation effects
CULTIVO DE CELULAS
CULTURE DE CELLULE
ECLAIRAGE
fed-batch culture
FEED ADDITIVES
Fundamental and applied biological sciences. Psychology
Haematococcus pluvialis
ILUMINACION
Light
LIGHTING
METHODE
METHODS
METODOS
XANTHOPHYLLE
XANTHOPHYLLS
XANTOFILAS
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Summary:To increase the cell concentration and the accumulation of astaxanthin, the effects of the fed-batch addition of 10-fold-concentrated medium to supply nutrients, as well as illumination with blue light emitting diodes (LEDs), on cell growth and accumulation of astaxanthin were studied for the cultivation of Haematococcus pluvialis. Using the fed-batch addition method, the cell concentration increased above 1 mg-dry cell/cm 3, and under illumination with blue LEDs, the astaxanthin concentration reached approximately 70 μg/cm 3. This method was much simpler to operate than the medium replacement method in operation and enabled us to attain a higher total yield of astaxanthin.
ISSN:1389-1723
1347-4421
DOI:10.1263/jbb.100.339