Interactions of macaque blastocysts with epithelial cells in vitro

BACKGROUND: Early in vitro studies of blastocyst formation in several primate species have demonstrated the feasibility of such studies. Initial studies of in vitro-fertilized oocytes cultured with buffalo rat liver cells suggested that other epithelial cells might be used to assess blastocyst adher...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2005-11, Vol.20 (11), p.3026-3032
Main Authors: Enders, Allen C., Meyers, Stuart, VandeVoort, Catherine A., Douglas, Gordon C.
Format: Article
Language:English
Subjects:
animal model
Animals
Biological and medical sciences
Blastocyst - drug effects
Blastocyst - physiology
Blastocyst - ultrastructure
Cell Adhesion
Cell Line
Cell Line, Tumor
Coculture Techniques
Collagen - pharmacology
Dogs
Drug Combinations
Epithelial Cells - physiology
Female
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
Humans
implantation
Laminin - pharmacology
Macaca mulatta
MDCK
Medical sciences
Microscopy, Electron
Proteoglycans - pharmacology
Rats
Rats, Inbred BUF
trophoblast
Trophoblasts - physiology
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Summary:BACKGROUND: Early in vitro studies of blastocyst formation in several primate species have demonstrated the feasibility of such studies. Initial studies of in vitro-fertilized oocytes cultured with buffalo rat liver cells suggested that other epithelial cells might be used to assess blastocyst adherence and penetration in vitro. METHODS: Macaque blastocysts were incubated with different epithelial cell lines or with Matrigel. The interaction was studied using light and transmission electron microscopy. RESULTS: In general, zona-free blastocysts attached 2 days after placing on the substrates. MDCK cells provided optimal conditions for blastocyst development. The best preparations showed some development of an amniotic cavity and distribution of cytotrophoblast and syncytial trophoblast. Distribution of syncytial trophoblast at the margin of the site and cytotrophoblast centrally was similar to that seen at the trophoblastic plate stage in this species. However, there was less syncytial trophoblast than is normally found at this stage, and total time from fertilization to the trophoblastic plate stage was delayed 2 days. CONCLUSIONS: While in vitro studies with blastocysts cannot completely mimic the intrauterine environment, they can illustrate some of the potential interactions and provide a situation in which parameters may be manipulated.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/dei178