Sensitivity improvement in capillary electrophoresis using organo-aqueous separation buffers and thermal lens detection

It is shown that organo-aqueous separation buffers show much promise when used in capillary electrophoresis separations with photothermal (thermal lens) detection systems. Acetonitrile-water and methanol-water mixtures were selected, as conventionally used in capillary electrophoresis. It is shown t...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2006-08, Vol.385 (8), p.1492-1503
Main Authors: Bendrysheva, Svetlana N, Proskurnin, Mikhail A, Pyell, Ute, Faubel, Werner
Format: Article
Language:English
Subjects:
Acetonitrile
Aqueous solutions
Binary mixtures
Buffers
Capillary electrophoresis
Electrophoresis
Sensitivity
Separation
Thermal lensing
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Summary:It is shown that organo-aqueous separation buffers show much promise when used in capillary electrophoresis separations with photothermal (thermal lens) detection systems. Acetonitrile-water and methanol-water mixtures were selected, as conventionally used in capillary electrophoresis. It is shown that, despite more sophisticated experimental conditions (significant heat outflow from the capillary body) and peak detection, the theoretical ratio of the thermal lens signal for a binary mixture to the thermal lens signal for an aqueous solution (or the corresponding ratio obtained experimentally under bulk batch conditions) can be used to predict the sensitivity of thermal lens detection in capillary electrophoresis. The limits of detection for 2-, 3-, and 4-nitrophenols selected as model compounds in 70% v/v acetonitrile separation buffers are 1 x 10(-6) M, 1 x 10(-6) M and 3 x 10(-7) M, respectively, and are therefore decreased by a factor of six compared to thermal lens detection in aqueous separation buffers. The overall increase in the thermal lens detection sensitivity in a 100% ACN buffer is a factor of 13.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-006-0602-3