Sophorolipid produced from the new yeast strain Wickerhamiella domercqiae induces apoptosis in H7402 human liver cancer cells

The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin,...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2006-08, Vol.72 (1), p.52-59
Main Authors: Chen, Jing, Song, Xin, Zhang, Hui, Qu, Yin-bo, Miao, Jun-ying
Format: Article
Language:English
Subjects:
Antineoplastic Agents - metabolism
Antineoplastic Agents - pharmacology
Apoptosis
Ascomycota - metabolism
Biological and medical sciences
Biotechnology
Calcium - analysis
Cancer
Cancer therapies
Caspase 3 - analysis
Cell cycle
Cell Line, Tumor
Cell Survival
Chromatin - metabolism
Colorimetry
Cytoplasm - chemistry
DNA Fragmentation
Fluorometry
Fundamental and applied biological sciences. Psychology
G1 Phase - drug effects
Glycolipids - biosynthesis
Glycolipids - pharmacology
Humans
In Situ Nick-End Labeling
Liver cancer
Liver Neoplasms - drug therapy
Liver Neoplasms - pathology
Microscopy, Electron, Transmission
Microscopy, Phase-Contrast
S Phase - drug effects
Wickerhamiella
Yeasts
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Summary:The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin, nuclear fragmentation, and appearance of apoptotic bodies, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive cells were stained dark brown. Sophorolipid treatment induced apoptosis in H7402 cells by blocking cell cycle at G1 phase and partly at S phase, activating caspase-3, and increasing Ca2+ concentration in cytoplasm. These findings may suggest a potential use of sophorolipid for liver cancer treatment.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-005-0243-z