The Effect of G-CSF in an Experimental MRSA Graft Infection in Mice

• Published in: Journal of investigative surgery 2005-09, Vol.18 (5), p.227-231
• Main Authors: Agalar, Canan, Eroglu, Erol, Sari, Mustafa, Sari, Aliye, Daphan, Cagatay, Agalar, Fatih
• Format: Article
• Language: English
• Subjects: Animals; foreign-body infection; G-CSF; Granulocyte Colony-Stimulating Factor - therapeutic use; Leukocyte Count; Male; Methicillin Resistance; Mice; MRSA; Prosthesis Implantation - adverse effects; Staphylococcal Infections - drug therapy; Staphylococcus aureus - drug effects; Surgical Wound Infection - drug therapy; Teicoplanin
• ISSN: 0894-1939; 1521-0553
• DOI: 10.1080/08941930500248581

Wound infection after prosthetic material implantation is a troublesome complication with an incidence of 2% to 10%. The effect of granulocyte colony-stimulating factor (G-CSF) was studied in an experimental methicillin-resistant Staphylococcus aureus (MRSA) graft infection model. Eighty adult mice were used. Under general anesthesia an abdominal incision of 2 cm in length was performed. A subcutaneous cavity of 2 × 2 cm in size was created. Polypropylene mesh pieces of 2 × 1 cm and MRSA solution of 0.1ml of 108 CFU/mL were used. G-CSF was applied systemically or locally in a dosage of 0.02 MU/30 g body weight. There were 8 groups: group I, wound + MRSA; group II, wound + mesh + MRSA; group III, wound + mesh + MRSA + G-CSF (ip, 48 h before operation); group IV, wound + mesh + MRSA + G-CSF (ip, 24 h before operation); group V, wound + mesh + MRSA + G-CSF (locally, into the cavity); group VI, wound + mesh (incubated in G-CSF solution for 4 h) + MRSA; group VII, wound + mesh + MRSA + G-CSF, ip, 24 h from operation; and group VIII (positive control group), wound + mesh + MRSA + Teicoplanin (0.03 mg/30 g body weight, ip, 1/2 h before operation). Three days after, animals were killed and incisions were examined for possible infection or abscess formation and wound failure. Meshes were removed; after vortexing and dilution, samples were incubated with 5% agar media. Results of bacterial incubation were evaluated 24 h and 48 h later. There were symptoms of wound infection and abscess formation in all groups except group VIII. In group VIII, MRSA was isolated in 7 events with a colony count below 103. Bacterial counts were above 106 (106-108) in all other groups. Thus, it was observed that wound infection could be created with this model, but G-CSF could not prevent the development of wound infection, whether it was administered systemically or locally. Teicoplanin decreased the number of colony-forming units of MRSA, and prevents wound infection in this MRSA wound infection model.