A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis

A simple and universally applicable protocol for extracting high‐quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of prote...

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Bibliographic Details
Published in:Electrophoresis 2006-07, Vol.27 (13), p.2782-2786
Main Authors: Wang, Wei, Vignani, Rita, Scali, Monica, Cresti, Mauro
Format: Article
Language:English
Subjects:
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel - methods
Plant Proteins - isolation & purification
Plant tissue
Plants - chemistry
Proteomics
Sample preparation
Silver Staining
Two-dimensional electrophoresis
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Summary:A simple and universally applicable protocol for extracting high‐quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200500722