Cell-free expression systems for eukaryotic protein production

Following the success of genome sequencing projects, attention has now turned to studies of the structure and function of proteins. Although cell-based expression systems for protein production have been widely used, they have certain limitations in terms of the quality and quantity of the proteins...

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Bibliographic Details
Published in:Current opinion in biotechnology 2006-08, Vol.17 (4), p.373-380
Main Authors: Endo, Yaeta, Sawasaki, Tatsuya
Format: Article
Language:English
Subjects:
Animals
Biotechnology - methods
Cell-Free System - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
Humans
Proteomics - methods
Recombinant Proteins - biosynthesis
Recombinant Proteins - isolation & purification
Reticulocytes - metabolism
Transcription, Genetic - genetics
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Summary:Following the success of genome sequencing projects, attention has now turned to studies of the structure and function of proteins. Although cell-based expression systems for protein production have been widely used, they have certain limitations in terms of the quality and quantity of the proteins produced and for high-throughput production. Many of these limitations can be circumvented by the use of cell-free translation systems. Among such systems, the wheat germ based system is of special interest for its eukaryotic nature; it has the significant advantage of producing eukaryotic multidomain proteins in a folded state. Several advances in the use of cell-free expression systems have been made in the past few years and successful applications of these systems to produce proteins for functional and structural biology studies have been reported.
ISSN:0958-1669
1879-0429
DOI:10.1016/j.copbio.2006.06.009