Fed-batch bioreactor production of mannosylerythritol lipids secreted by Pseudozyma aphidis

Two strains of Pseudozyma aphidis, DSM 70725 and DSM 14930, were used for the bioreactor production of mannosylerythritol lipids (MELs). Foam formation interfered substantially with the cultivation process. Soybean oil was simultaneously employed as both carbon source and anti-foam agent. Primary ME...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2005-09, Vol.68 (5), p.607-613
Main Authors: Rau, U, Nguyen, L. A, Roeper, H, Koch, H, Lang, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomass
Bioreactors
Bioreactors - microbiology
Biotechnology
Carbon sources
Chemical reactors
Culture Media
Fermentation
Fundamental and applied biological sciences. Psychology
Glycolipids - biosynthesis
Lipids
Nitrates
Nitrogen - metabolism
Pseudozyma
Pseudozyma aphidis
Soybean oil
Soybean Oil - metabolism
Soybeans
Studies
Ustilaginales - chemistry
Ustilaginales - metabolism
Yeasts
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Summary:Two strains of Pseudozyma aphidis, DSM 70725 and DSM 14930, were used for the bioreactor production of mannosylerythritol lipids (MELs). Foam formation interfered substantially with the cultivation process. Soybean oil was simultaneously employed as both carbon source and anti-foam agent. Primary MEL formation occurred after nitrate limitation. After a first short time-period of nitrate limitation and further nitrate addition, MELs were secreted in spite of nitrate excess. The sedimentation of MEL-enriched beads indicated enhanced product formation. Maximum yield, productivity and yield coefficient of 165 g l-¹, 13.9 g l-¹ day-¹ and 0.92 g g-¹ were achieved using strain DSM 14930 with additional substrate-feeding (glucose, sodium nitrate, yeast extract) and a foam-controlled soybean oil supply.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-005-1906-5