Ceftiofur Derivatives in Serum, Uterine Tissues, Cotyledons, and Lochia after Fetal Membrane Retention

• Published in: Journal of dairy science 2006-09, Vol.89 (9), p.3431-3438
• Main Authors: Drillich, M., Arlt, S., Kersting, S., Bergwerff, A.A., Scherpenisse, P., Heuwieser, W.
• Format: Article
• Language: English
• Subjects: Animal productions; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - blood; Anti-Bacterial Agents - pharmacokinetics; antibiotic residues; Arcanobacterium pyogenes; Biological and medical sciences; blood serum; Body Temperature; Cattle - metabolism; Cattle Diseases - drug therapy; Cattle Diseases - metabolism; ceftiofur; Cephalosporins - administration & dosage; Cephalosporins - blood; Cephalosporins - pharmacokinetics; chemical concentration; dairy cows; endometrium; Endometrium - chemistry; Endometrium - metabolism; Escherichia coli; Extraembryonic Membranes - chemistry; Extraembryonic Membranes - metabolism; Female; Food industries; Fundamental and applied biological sciences. Psychology; Fusobacterium necrophorum; Injections, Subcutaneous - veterinary; Milk and cheese industries. Ice creams; minimum inhibitory concentration; pharmacokinetics; placenta; Placenta - chemistry; Placenta - metabolism; Placenta, Retained - drug therapy; Placenta, Retained - metabolism; Placenta, Retained - veterinary; Pregnancy; retained fetal membrane; retained placenta; Serum - chemistry; Terrestrial animal productions; Time Factors; Vertebrates
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(06)72380-3

The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.