Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex

We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5 -GAGTC/5 -GACTC) occurs when this protein is added to the reactio...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Moscow) 2006-07, Vol.71 (7), p.815-820
Main Authors: Yunusova, A K, Rogulin, E A, Artyukh, R I, Zheleznaya, L A, Matvienko, N I
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Bacteria
Bacteriology
Deoxyribonuclease I - genetics
Deoxyribonuclease I - metabolism
Dimerization
DNA Restriction Enzymes - genetics
DNA Restriction Enzymes - isolation & purification
DNA Restriction Enzymes - metabolism
Enzymes
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Genetic code
Models, Genetic
Molecular Sequence Data
Nucleotide sequence
Open Reading Frames
Protein Subunits - metabolism
Reading
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5 -GAGTC/5 -GACTC) occurs when this protein is added to the reaction mixture containing N.BspD6I. The protein encoded by the open reading frame and the nickase are suggested to be subunits of heterodimeric restriction endonuclease R.BspD6I.
ISSN:0006-2979
1608-3040
0320-9725
DOI:10.1134/S0006297906070157