On the preparation, microscopic investigation and application of ISCOMs

ISCOM matrices constitute colloidal structures formed from Quillaja saponins, cholesterol and phospholipid. Addition of protein antigens to these matrices leads to the formation of ISCOMs. In this review we report on microscopic investigations of ISCOM matrices and ISCOMs as well as related colloida...

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Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2006-01, Vol.37 (8), p.724-734
Main Authors: Myschik, Julia, Lendemans, Dirk G., McBurney, Warren T., Demana, Patrick H., Hook, Sarah, Rades, Thomas
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - chemistry
Adjuvants, Immunologic - physiology
Animals
Antigens - chemistry
Chemistry, Pharmaceutical
Cholesterol
Colloids - analysis
Colloids - chemistry
Helices
Humans
ISCOMs
ISCOMs - chemistry
ISCOMs - immunology
ISCOMs - ultrastructure
Lamellae structures
Microscopy
Microscopy, Electron
Phospholipids
Quil-A
Quillaja Saponins
Ring-like micelles
Saponins
Structure-Activity Relationship
Worm-like micelles
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Summary:ISCOM matrices constitute colloidal structures formed from Quillaja saponins, cholesterol and phospholipid. Addition of protein antigens to these matrices leads to the formation of ISCOMs. In this review we report on microscopic investigations of ISCOM matrices and ISCOMs as well as related colloidal structures, such as helices, worm-like micelles, ring-like micelles, and lamellae structures. We briefly outline the immunologic basis for the use of ISCOMs as vaccine delivery systems, and describe the various methods to form ISCOMs. Negative staining transmission electron micrographs of all colloidal structures are presented and described. On the basis of our microscopic investigations, different formation mechanisms of ISCOMS are discussed.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2006.03.016