Biolistic inoculation of soybean plants with soybean dwarf virus

Soybean dwarf virus (SbDV), a member of the genus Luteovirus, has been transmitted only by aphid vectors. The possibility of using a biolistic procedure of transmitting SbDV to soybean plants without relying on aphid vectors was investigated. Biolistic inoculation using the Helios Gene Gun System wi...

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Bibliographic Details
Published in:Journal of virological methods 2006-10, Vol.137 (1), p.164-167
Main Authors: Yamagishi, Noriko, Terauchi, Hidetaka, Kanematsu, Seiji, Hidaka, Soh
Format: Article
Language:English
Subjects:
Biolistics - methods
Biological and medical sciences
DNA, Complementary
Fundamental and applied biological sciences. Psychology
Glycine max - virology
Luteoviridae
Luteovirus
Luteovirus - genetics
Luteovirus - physiology
Mechanical inoculation
Microbiology
Particle bombardment
Phloem-limited virus
Plant Leaves - virology
RNA, Viral - analysis
SbDV
Soybean dwarf virus
Techniques used in virology
Virology
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Summary:Soybean dwarf virus (SbDV), a member of the genus Luteovirus, has been transmitted only by aphid vectors. The possibility of using a biolistic procedure of transmitting SbDV to soybean plants without relying on aphid vectors was investigated. Biolistic inoculation using the Helios Gene Gun System with RNA transcribed in vitro from a full-length cDNA clone of the DS strain of SbDV (pSV-DS) resulted in 1/12 to 3/13 soybean plants infected systemically. The infected soybean plants showed the characteristic symptoms of SbDV-DS within 6 weeks after inoculation and the accumulation of SbDV-specific RNA species such as genomic and subgenomic RNAs in the upper non-inoculated leaves. The progeny virus derived from RNA transcribed in vitro from pSV-DS could be transmitted by aphid vectors, as is the case with native SbDV-DS. This is the first report of direct inoculation of soybean plants with SbDV without using aphid vectors.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2006.06.001