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Biolistic inoculation of soybean plants with soybean dwarf virus

Soybean dwarf virus (SbDV), a member of the genus Luteovirus, has been transmitted only by aphid vectors. The possibility of using a biolistic procedure of transmitting SbDV to soybean plants without relying on aphid vectors was investigated. Biolistic inoculation using the Helios Gene Gun System wi...

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Published in:	Journal of virological methods 2006-10, Vol.137 (1), p.164-167
Main Authors:	Yamagishi, Noriko, Terauchi, Hidetaka, Kanematsu, Seiji, Hidaka, Soh
Format:	Article
Language:	English
Subjects:	Biolistics - methods Biological and medical sciences DNA, Complementary Fundamental and applied biological sciences. Psychology Glycine max - virology Luteoviridae Luteovirus Luteovirus - genetics Luteovirus - physiology Mechanical inoculation Microbiology Particle bombardment Phloem-limited virus Plant Leaves - virology RNA, Viral - analysis SbDV Soybean dwarf virus Techniques used in virology Virology
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container_title	Journal of virological methods
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creator	Yamagishi, Noriko Terauchi, Hidetaka Kanematsu, Seiji Hidaka, Soh
description	Soybean dwarf virus (SbDV), a member of the genus Luteovirus, has been transmitted only by aphid vectors. The possibility of using a biolistic procedure of transmitting SbDV to soybean plants without relying on aphid vectors was investigated. Biolistic inoculation using the Helios Gene Gun System with RNA transcribed in vitro from a full-length cDNA clone of the DS strain of SbDV (pSV-DS) resulted in 1/12 to 3/13 soybean plants infected systemically. The infected soybean plants showed the characteristic symptoms of SbDV-DS within 6 weeks after inoculation and the accumulation of SbDV-specific RNA species such as genomic and subgenomic RNAs in the upper non-inoculated leaves. The progeny virus derived from RNA transcribed in vitro from pSV-DS could be transmitted by aphid vectors, as is the case with native SbDV-DS. This is the first report of direct inoculation of soybean plants with SbDV without using aphid vectors.
doi_str_mv	10.1016/j.jviromet.2006.06.001
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subjects	Biolistics - methods Biological and medical sciences DNA, Complementary Fundamental and applied biological sciences. Psychology Glycine max - virology Luteoviridae Luteovirus Luteovirus - genetics Luteovirus - physiology Mechanical inoculation Microbiology Particle bombardment Phloem-limited virus Plant Leaves - virology RNA, Viral - analysis SbDV Soybean dwarf virus Techniques used in virology Virology
title	Biolistic inoculation of soybean plants with soybean dwarf virus
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