Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material

In this study, we describe a technique that allows for the production and the use of tissue microarrays (TMA) from HOPE-fixed, paraffin-embedded specimens. The combination of these two technologies unites the advantages of the high throughput aspects in TMA, with good preservation of nucleic acids,...

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Bibliographic Details
Published in:Pathology, research and practice research and practice, 2005-01, Vol.201 (8), p.599-602
Main Authors: Goldmann, Torsten, Drömann, Daniel, Marzouki, Marouane, Schimmel, Udo, Debel, Karsten, Branscheid, Detlev, Zeiser, Tobias, Rupp, Jan, Gerdes, Johannes, Zabel, Peter, Vollmer, Ekkehard
Format: Article
Language:English
Subjects:
Biomarkers
Biopsy
Female
Fixatives
HOPE-technique
Humans
Immunohistochemistry
Immunohistochemistry - methods
In Situ Hybridization
Molecular Biology
Paraffin
Paraffin Embedding
RNA, Messenger - metabolism
Tissue Array Analysis - methods
Tissue Fixation - methods
Tissue microarrays
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Summary:In this study, we describe a technique that allows for the production and the use of tissue microarrays (TMA) from HOPE-fixed, paraffin-embedded specimens. The combination of these two technologies unites the advantages of the high throughput aspects in TMA, with good preservation of nucleic acids, proteins, and morphology of HOPE-fixed tissues, thus substantially widening the capabilities presently available for molecular studies in paraffin-embedded tissues.
ISSN:0344-0338
1618-0631
DOI:10.1016/j.prp.2005.07.006