A silica-based monolithic column in capillary HPLC and CEC coupled with ESI-MS or electrospray-atmospheric-pressure laser ionization-MS

We describe the successful coupling of CEC and capillary HPLC with the recently developed atmospheric‐pressure laser ionization (APLI) method. APLI is suitable for selectively and sensitively ionizing nonpolar aromatic compounds at ambient pressure for subsequent mass‐selective detection. The polycy...

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Bibliographic Details
Published in:Electrophoresis 2005-11, Vol.26 (21), p.4098-4103
Main Authors: Droste, Stefan, Schellenträger, Marc, Constapel, Marc, Gäb, Siegmar, Lorenz, Matthias, Brockmann, Klaus J., Benter, Thorsten, Lubda, Dieter, Schmitz, Oliver J.
Format: Article
Language:English
Subjects:
Atmospheric-pressure laser ionization
CEC
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Equipment Design
Mass spectrometry
Monolithic column
Polycyclic Aromatic Hydrocarbons - isolation & purification
Silicon Dioxide
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:We describe the successful coupling of CEC and capillary HPLC with the recently developed atmospheric‐pressure laser ionization (APLI) method. APLI is suitable for selectively and sensitively ionizing nonpolar aromatic compounds at ambient pressure for subsequent mass‐selective detection. The polycyclic aromatic hydrocarbons used as analytes are first separated either by CEC on a silica‐based monolithic column or by capillary HPLC. The eluent, along with a sheath flow, is volatilized by microelectrospray and then selectively ionized by excimer laser (KrF*) radiation via two‐photon excitation. A QTOF‐MS is used as mass‐selective detector. This interface combination makes soft ionization of thermally labile nonpolar aromatic analytes possible.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200500326