Enhancement of the Transmesothelial Resistance of the Parietal Sheep Peritoneum by Epinephrine In Vitro: Ussing-type Chamber Experiments

:  The peritoneal mesothelium constitutes an ion transport barrier that is taken advantage of in peritoneal dialysis. The aim of this study was to investigate the effects of epinephrine on the electrical transmesothelial resistance (RTM) of the isolated parietal sheep peritoneum by means of Ussing‐t...

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Bibliographic Details
Published in:Artificial organs 2005-11, Vol.29 (11), p.919-922
Main Authors: Stefanidis, Ioannis, Zarogiannis, Sotirios, Hatzoglou, Chrissi, Liakopoulos, Vasilios, Kourti, Panagiota, Poultsidi, Antigoni, Mertens, Peter R., Gourgoulianis, Konstantinos, Molyvdas, Paschalis-Adam
Format: Article
Language:English
Subjects:
Animals
Bioreactors
Electric Impedance
Electrochemistry - instrumentation
Electrochemistry - methods
Epinephrine
Epinephrine - administration & dosage
Female
In Vitro Techniques
Male
Peritoneum
Peritoneum - drug effects
Peritoneum - physiology
Permeability
Permeability - drug effects
Sheep
Transmesothelial resistance
Ussing-type chamber
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Summary::  The peritoneal mesothelium constitutes an ion transport barrier that is taken advantage of in peritoneal dialysis. The aim of this study was to investigate the effects of epinephrine on the electrical transmesothelial resistance (RTM) of the isolated parietal sheep peritoneum by means of Ussing‐type chamber experiments. Intact parietal (diaphragmatic) peritoneal samples were obtained from adult sheep immediately after sacrifice and transferred within 0.5 h to the laboratory in a cooled Krebs–Ringer bicarbonate solution (4°C, pH 7.5), bubbled with 95% O2−5% CO2. A parietal peritoneal planar sheet was mounted in a Ussing‐type chamber. Epinephrine (10−7 M) was added to the apical and the basolateral side. The RTM was measured before and serially after the addition of epinephrine for 30 min. As active ion transport is temperature‐dependent, all measurements were performed at 37°C. The results were calculated as means with standard errors (x ± SE) of six independent experiments. The control RTM was 20.05 ± 0.61 Ω·cm2. The addition of epinephrine to the basolateral side within 1 min induced an increase of RTM to 21.8 ± 0.45 Ω·cm2, which decreased thereafter progressively to reach control values again after 15 min. A similar effect of epinephrine on the apical side was apparent with a rapid rise of RTM to 22.5 ± 0.66 Ω·cm2 and a subsequent decrease (P 
ISSN:0160-564X
1525-1594
DOI:10.1111/j.1525-1594.2005.00157.x